A REAL-TIME PCR-BASED ASSAY FOR DETECTION OF WUCHERERIA BANCROFTI DNA IN BLOOD AND MOSQUITOES
Identifieur interne : 007A60 ( Main/Exploration ); précédent : 007A59; suivant : 007A61A REAL-TIME PCR-BASED ASSAY FOR DETECTION OF WUCHERERIA BANCROFTI DNA IN BLOOD AND MOSQUITOES
Auteurs : Ramakrishna U. Rao ; Laura J. Atkinson ; Reda M. R. Ramzy ; Hanan Helmy ; Hoda A. Farid ; Moses. J. Bockarie ; Melinda Susapu ; Sandra J. Laney ; Steven A. Williams ; Gary J. WeilSource :
- The American journal of tropical medicine and hygiene [ 0002-9637 ] ; 2006.
Abstract
We developed and evaluated real-time polymerase chain reaction (PCR) assays for detecting
Url:
PubMed: 16687688
PubMed Central: 2196401
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 003332
- to stream Pmc, to step Curation: 003331
- to stream Pmc, to step Checkpoint: 003D04
- to stream Ncbi, to step Merge: 002228
- to stream Ncbi, to step Curation: 002228
- to stream Ncbi, to step Checkpoint: 002228
- to stream Main, to step Merge: 007C53
- to stream Main, to step Curation: 007A60
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A REAL-TIME PCR-BASED ASSAY FOR DETECTION OF <italic>WUCHERERIA BANCROFTI</italic> DNA IN BLOOD AND MOSQUITOES</title><author><name sortKey="Rao, Ramakrishna U" sort="Rao, Ramakrishna U" uniqKey="Rao R" first="Ramakrishna U." last="Rao">Ramakrishna U. Rao</name></author><author><name sortKey="Atkinson, Laura J" sort="Atkinson, Laura J" uniqKey="Atkinson L" first="Laura J." last="Atkinson">Laura J. Atkinson</name></author><author><name sortKey="Ramzy, Reda M R" sort="Ramzy, Reda M R" uniqKey="Ramzy R" first="Reda M. R." last="Ramzy">Reda M. R. Ramzy</name></author><author><name sortKey="Helmy, Hanan" sort="Helmy, Hanan" uniqKey="Helmy H" first="Hanan" last="Helmy">Hanan Helmy</name></author><author><name sortKey="Farid, Hoda A" sort="Farid, Hoda A" uniqKey="Farid H" first="Hoda A." last="Farid">Hoda A. Farid</name></author><author><name sortKey="Bockarie, Moses J" sort="Bockarie, Moses J" uniqKey="Bockarie M" first="Moses. J." last="Bockarie">Moses. J. Bockarie</name></author><author><name sortKey="Susapu, Melinda" sort="Susapu, Melinda" uniqKey="Susapu M" first="Melinda" last="Susapu">Melinda Susapu</name></author><author><name sortKey="Laney, Sandra J" sort="Laney, Sandra J" uniqKey="Laney S" first="Sandra J." last="Laney">Sandra J. Laney</name></author><author><name sortKey="Williams, Steven A" sort="Williams, Steven A" uniqKey="Williams S" first="Steven A." last="Williams">Steven A. Williams</name></author><author><name sortKey="Weil, Gary J" sort="Weil, Gary J" uniqKey="Weil G" first="Gary J." last="Weil">Gary J. Weil</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">16687688</idno><idno type="pmc">2196401</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196401</idno><idno type="RBID">PMC:2196401</idno><date when="2006">2006</date><idno type="wicri:Area/Pmc/Corpus">003332</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003332</idno><idno type="wicri:Area/Pmc/Curation">003331</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">003331</idno><idno type="wicri:Area/Pmc/Checkpoint">003D04</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">003D04</idno><idno type="wicri:Area/Ncbi/Merge">002228</idno><idno type="wicri:Area/Ncbi/Curation">002228</idno><idno type="wicri:Area/Ncbi/Checkpoint">002228</idno><idno type="wicri:doubleKey">0002-9637:2006:Rao R:a:real:time</idno><idno type="wicri:Area/Main/Merge">007C53</idno><idno type="wicri:Area/Main/Curation">007A60</idno><idno type="wicri:Area/Main/Exploration">007A60</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A REAL-TIME PCR-BASED ASSAY FOR DETECTION OF <italic>WUCHERERIA BANCROFTI</italic> DNA IN BLOOD AND MOSQUITOES</title><author><name sortKey="Rao, Ramakrishna U" sort="Rao, Ramakrishna U" uniqKey="Rao R" first="Ramakrishna U." last="Rao">Ramakrishna U. Rao</name></author><author><name sortKey="Atkinson, Laura J" sort="Atkinson, Laura J" uniqKey="Atkinson L" first="Laura J." last="Atkinson">Laura J. Atkinson</name></author><author><name sortKey="Ramzy, Reda M R" sort="Ramzy, Reda M R" uniqKey="Ramzy R" first="Reda M. R." last="Ramzy">Reda M. R. Ramzy</name></author><author><name sortKey="Helmy, Hanan" sort="Helmy, Hanan" uniqKey="Helmy H" first="Hanan" last="Helmy">Hanan Helmy</name></author><author><name sortKey="Farid, Hoda A" sort="Farid, Hoda A" uniqKey="Farid H" first="Hoda A." last="Farid">Hoda A. Farid</name></author><author><name sortKey="Bockarie, Moses J" sort="Bockarie, Moses J" uniqKey="Bockarie M" first="Moses. J." last="Bockarie">Moses. J. Bockarie</name></author><author><name sortKey="Susapu, Melinda" sort="Susapu, Melinda" uniqKey="Susapu M" first="Melinda" last="Susapu">Melinda Susapu</name></author><author><name sortKey="Laney, Sandra J" sort="Laney, Sandra J" uniqKey="Laney S" first="Sandra J." last="Laney">Sandra J. Laney</name></author><author><name sortKey="Williams, Steven A" sort="Williams, Steven A" uniqKey="Williams S" first="Steven A." last="Williams">Steven A. Williams</name></author><author><name sortKey="Weil, Gary J" sort="Weil, Gary J" uniqKey="Weil G" first="Gary J." last="Weil">Gary J. Weil</name></author></analytic><series><title level="j">The American journal of tropical medicine and hygiene</title><idno type="ISSN">0002-9637</idno><idno type="eISSN">1476-1645</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">We developed and evaluated real-time polymerase chain reaction (PCR) assays for detecting <italic>Wuchereria bancrofti</italic> DNA in human blood and in mosquitoes. An assay based on detection of the <italic>W. bancrofti</italic> “LDR” repeat DNA sequence was more sensitive than an assay for <italic>Wolbachia</italic> 16S rDNA. The LDR-based assay was sensitive for detecting microfilarial DNA on dried membrane filters or on filter paper. We also compared real-time PCR with conventional PCR (C-PCR) for detecting <italic>W. bancrofti</italic> DNA in mosquito samples collected in endemic areas in Egypt and Papua New Guinea. Although the two methods had comparable sensitivity for detecting filarial DNA in reference samples, real-time PCR was more sensitive than C-PCR in practice with field samples. Other advantages of real-time PCR include its high-throughput capacity and decreased risk of cross-contamination between test samples. We believe that real-time PCR has great potential as a tool for monitoring progress in large-scale filariasis elimination programs.</p></div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Atkinson, Laura J" sort="Atkinson, Laura J" uniqKey="Atkinson L" first="Laura J." last="Atkinson">Laura J. Atkinson</name><name sortKey="Bockarie, Moses J" sort="Bockarie, Moses J" uniqKey="Bockarie M" first="Moses. J." last="Bockarie">Moses. J. Bockarie</name><name sortKey="Farid, Hoda A" sort="Farid, Hoda A" uniqKey="Farid H" first="Hoda A." last="Farid">Hoda A. Farid</name><name sortKey="Helmy, Hanan" sort="Helmy, Hanan" uniqKey="Helmy H" first="Hanan" last="Helmy">Hanan Helmy</name><name sortKey="Laney, Sandra J" sort="Laney, Sandra J" uniqKey="Laney S" first="Sandra J." last="Laney">Sandra J. Laney</name><name sortKey="Ramzy, Reda M R" sort="Ramzy, Reda M R" uniqKey="Ramzy R" first="Reda M. R." last="Ramzy">Reda M. R. Ramzy</name><name sortKey="Rao, Ramakrishna U" sort="Rao, Ramakrishna U" uniqKey="Rao R" first="Ramakrishna U." last="Rao">Ramakrishna U. Rao</name><name sortKey="Susapu, Melinda" sort="Susapu, Melinda" uniqKey="Susapu M" first="Melinda" last="Susapu">Melinda Susapu</name><name sortKey="Weil, Gary J" sort="Weil, Gary J" uniqKey="Weil G" first="Gary J." last="Weil">Gary J. Weil</name><name sortKey="Williams, Steven A" sort="Williams, Steven A" uniqKey="Williams S" first="Steven A." last="Williams">Steven A. Williams</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 007A60 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 007A60 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:2196401
|texte= A REAL-TIME PCR-BASED ASSAY FOR DETECTION OF WUCHERERIA BANCROFTI DNA IN BLOOD AND MOSQUITOES
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:16687688" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |